报告题目：Design of the next-generation two-photon microscope and tissue cutting instrument for whole organ imaging
时间：5月11日下午14：30
地点：武汉光电国家实验室A101
报告人：Shih-Chi Chen，Assistant Professor, Department of Mechanical and Automation Engineering
邀请人：骆清铭教授
Abstract:
I will present our recent work on femtosecond laser manipulation based on digital micromirror devices (DMD) and binary hologram; and their applications in developing the next-generation two-photon and random-access microscopes. The DMD-based pulse/beam shaper can arbitrarily shape a femtosecond laser at a speed of up to 30 MHz and 32 kHz respectively, around 3 orders of magnitude faster than existing methods. In the second part, I will present the development of a flexure-based vibrating blade microtome for sectioning soft tissues with submicron level flatness and precision. Cutting mechanism of soft tissues is modeled based on fracture mechanics in order to establish quantitative relationships between the sectioning quality, i.e. flatness, and microtome operation parameters, including vibration frequency, amplitude, and sample feed rate. The precision microtome can be integrated with the two-photon microscope to generate volumetric image of an entire organ, such as a mouse brain or liver, with subcellular resolution.
Biographical Sketch:
Prof. Shih-Chi Chen received his B.S. degree in Power Mechanical Engineering from the National Tsing Hua University, Taiwan, in 1999. He received his S.M. and Ph.D. degrees in Mechanical Engineering from the Massachusetts Institute of Technology, Cambridge, in 2003 and 2007, respectively. Following his graduate work, he entered a post-doctoral fellowship in the Wellman Center for Photomedicine, Harvard Medical School, where his research was focused on biomedical optics and endomicroscopy. Before joining CUHK, he was a Senior Scientist at Nano Terra, Inc., a start-up company founded by Prof. George Whitesides at Harvard University, to develop novel methods and precision instruments for the control of various interface functionalities and soft lithography. His current research interests include precision engineering, laser optics, MEMS, and nanomanufacturing. Prof. Chen is a member of the following professional societies: ASME, ASPE, SPIE, IEEE, and OSA. He is the recipient of a 2003 R&D 100 Award for the design of a microscale six-axis nanopositioner.
